Interactions with mycorrhizal fungi in two closely related hybridizing orchid species

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Interactions with mycorrhizal fungi in two closely related hybridizing orchid species

The nuclear ribosomal DNAwas used to identify the orchid mycorrhizal fungi found in roots of Orchis xbivonae and its parental species Orchis anthropophora and Orchis italica. Polymerase chain reaction products were sequenced and identified using the expanded database. We determined that closely related Tulasnellaceae are mycorrhizal in the three orchid taxa, suggesting that the mycorrhizal part...

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Rapid Mitochondrial Genome Evolution through Invasion of Mobile Elements in Two Closely Related Species of Arbuscular Mycorrhizal Fungi

Arbuscular mycorrhizal fungi (AMF) are common and important plant symbionts. They have coenocytic hyphae and form multinucleated spores. The nuclear genome of AMF is polymorphic and its organization is not well understood, which makes the development of reliable molecular markers challenging. In stark contrast, their mitochondrial genome (mtDNA) is homogeneous. To assess the intra- and inter-sp...

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Mycorrhizal Fungal Diversity and Community Composition in Two Closely Related Platanthera (Orchidaceae) Species

While it is generally acknowledged that orchid species rely on mycorrhizal fungi for completion of their life cycle, little is yet known about how mycorrhizal fungal diversity and community composition vary within and between closely related orchid taxa. In this study, we used 454 amplicon pyrosequencing to investigate variation in mycorrhizal communities between pure (allopatric) and mixed (sy...

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Fungal Diversity A novel technique for isolating orchid mycorrhizal fungi

We describe a technique for isolating mycorrhizal fungi from roots of orchids. This technique involves selection and treatment of roots, preparation of pelotons, treatment of pelotons, culture of pelotons so that fungal hyphae grow out and strain purification. The technique is considered better because 1) problems of fungal and bacterial contamination are resolved, 2) endophytic bacteria are su...

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Differentiation of two closely related species of the genus Empoasca (Hem.: Cicadellidae)

پیش‌تر، گونه‌ای زنجرک از مزارع چغندرقند استان اصفهان به اشتباه به نام Empoasca decipiens (Paoli) گزارش شده است که در این نوشتار نام صحیح آن به‌صورت Empoasca meridiana Zachvatkin اعلام می‌گردد. گونه‌ی اخیر دارای سینونیم شناخته‌تری به نام Empoasca punjabensis Singh-Pruthi می‌باشد. گونه‌های آسیایی خویشاوند گونه‌ی E. decipiens، براساس شکل پیگوفر (pygofer) ژنیتالیای نر ازهم تفکیک می‌گردند، به‌طوری‌ک...

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ژورنال

عنوان ژورنال: Acta Botanica Croatica

سال: 2014

ISSN: 0365-0588

DOI: 10.2478/botcro-2013-0028